These CRISPR transfection protocols describe how to transfect cultured cells with ribonucleoprotein (RNP) complexes consisting of purified Cas9 nuclease duplexed with synthetic guide RNA (synthetic sgRNA or annealed crRNA and tracrRNA).
Download these 3 CRISPR transfection protocols and you'll learn how to utilize CRISPR transfection techniques that enable optimal delivery of CRISPR components to your cells, resulting in high-efficiency CRISPR genome edits. Such techniques include:
- Lipofection Transfection
- Electroporation Transfection
- Nucleofection Transfection